Comparison of spectroscopical, chromatographical and immunological methods for detection of mycotoxin content and fungus attack in cereal samples
Description:
Support of breeding methodical processes that serve the reduction of the mycotoxin content in wheat and other cereals in the event of an attack of ear fusarioses. In closed cooperation with other institutions (FAL, BBA) belonging to the ministry of agriculture and consumer protection (BMELV) new efficient HPLC/MS and IR rapid methods for the detection of relevant mycotoxins in grains, comparison with immunological methods (enzyme immunoassays, EIAs) for the determination of mycotoxin contents (competitive EIAs) and quantity of mycelium (non-competitive EIAs) will be developed. The spectroscopic methods will be applied in the near infrared range (NIR) as well as in the mid infrared range (MIR). It is assumed that these rapid methods will be fruitfully applied in the context of breeding research, individual aspects of cultivation and quality control. The methods shall be evaluated with regard to the comparability of their results and their use for a screening in plant breeding.
Executive Institute:
Institute for Ecological Chemistry, Plant Analysis and Stored Products Protection (JKI - ÖPV) Details of Institute for Ecological Chemistry, Plant Analysis and Stored Products Protection
Parent institution:
Federal Research Centre for Cultivated Plants – Julius Kühn-Institut (JKI) Details of (JKI) (Saxony-Anhalt)
Contract period:
01. 02. 2006 - 31. 01. 2008
Participating Institutions:
- Institute of Resistance Research and Stress Tolerance (JKI-RS) Details of JKI-RS
- University of Hohenheim (UH) Details of UH
- State Plant Breeding Institute (720) (LSA) Details of LSA
Funding Programme:
- BMELV / Core funding Details of BMELV / Core funding
Subject:
- Plant Breeding
- Biotechnology
Purpose of research:
Basic research
Funding Institutions:
- Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) Details of (BMELV)
Project Management Agency:
- Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) Details of BMELV