Collaborative project ESBL- and (fluoro-)quinolone resitance in enterobactericeae (RESET). Project IP1 characterization of newly emerging mechanisms for transfer of resistance to ß-lactam antibiotics with extended spectrum (ESBLs) and (fluoro) quinolones in German-Salmonella enterica and Escherichia coli isolates from healthy poultry and pigs and products thereof
Description:
The collaborative project aims at assessing the impact of different origins, transmission routes and pathogen attributes on the risk for humans being exposed to ESBL-producing and (fluoro)quinolone-resistant Enterobacteriaceae. Thus, most research activities will take place in areas of possible pathogen sources (animals, food) which may have an impact on human health. Existing data and isolates from studies and surveillance activities will be completed and supplemented by epidemiological studies and diagnostic investigations. Combining reservoir-related (including humans), pathogen-related and epidemiological information will contribute to quantify and assess risks which can be integrated in the development of control strategies under DART (German strategy antibiotic resistance). The first aspect will be molecular-based studies on the prevalence of ESBL genes as well as plasmid-mediated (fluoro)quinolone resistance (PMQR) genes in E. coli and S. enterica to highlight which factors play a role for their selection, stable maintenance and dissemination. Typing and detailed characterisation of strains carrying such genes will be conducted to differentiate between the clonal spread of resistant strains and the horizontal transfer of resistance genes. Secondly, the quantitative occurrence of ESBL- and PMQR-carrying E. coli and S. enterica in animal, food and human populations and in their environment will be analysed. Finally, a risk assessment concept will be generated based on the available knowledge. Moreover, a source attribution approach will be developed to calculate the attributable fraction of the different sources considered in the exposure assessment. Based on these results, recommendations will be given to improve the control of ESBL- and PMQR-carrying E. coli and S. enterica in Germany. Such evidence-based recommendations may form the basis for a general concept of control strategies of resistant bacteria in Germany.
Executive Institute:
BfR - Department 4: Biological Safety (BfR - BIOS) Details of BfR - Department 4: Biological Safety
Parent institution:
Federal Institute for Risk Assessment (BfR) Details of (BfR) (Berlin)
Contract period:
01. 11. 2010 - 31. 10. 2013
Participating Institutions:
- Institute of Applied Microbiology Details of Institute of Applied Microbiology
- Institut für Biometrie, Epidemiologie und Informationsverarbeitung (IBEI) Details of IBEI
- Department of Pharmacology, Toxicology & Pharmacy Details of Department of Pharmacology, Toxicology & Pharmacy
- National Reference Center for Staphylococci (NRZ) Details of NRZ
- Institute for Animal Hygiene and Environmental Hygiene Details of Institute for Animal Hygiene and Environmental Hygiene
- Institute for Hygiene and Environmental Medicine Details of Institute for Hygiene and Environmental Medicine
- Department of Chemistry Details of Department of Chemistry
Collaborative Project:
Network ESBL and (fluoro)quinolone resistance in Enterobacteriaceae (RESET): Resistance in animals and humans Details of Network ESBL and (fluoro)quinolone resistance in Enterobacteriaceae (RESET): Resistance in animals and humans
Funding Programme:
- BMBF Funding Details of BMBF Funding
Subject:
- Animal health
Purpose of research:
Applied research
Funding Institutions:
- Federal Ministry of Education and Research (BMBF) Details of (BMBF)
Project Management Agency:
- Federal Ministry of Education and Research (BMBF) Details of BMBF