Enzymatic rendering for prion-free animal products.
Test the hypothesis that the procedure for enzymatic digestion as worked out in the paper of Langeveld et al. (2003) is effective in removing infectivity (preferably at least 5 orders of magnitude) from bovine brain stem material infected with bovine spongiform encephalopathy (BSE). Since the digestion is preceded by an essential pretreatment of heating at 115 oC the effect of this pretreatment will also be measured. Titers will be determined by end-point titration. Transgenic mice with boPrP gene (boTgXV, Buschmann et al. 2000) are suitable for this, since they can reach titers of 8 (10log value).
FLI - Institute for Novel and Emerging Infectious Diseases (FLI-INNT) Details of FLI - Institute for Novel and Emerging Infectious Diseases
Friedrich-Loeffler-Institute - Federal Research Institute for Animal Health (FLI) Details of (FLI) (Mecklenburg-Vorpommern)
01. 10. 2005 - 31. 10. 2009
- Central Veterinary Institute of Wageningen UR Details of Central Veterinary Institute of Wageningen UR
- Animal health
Purpose of research: