Optical probe technique for the assessment of multidrug resistance profiles in 3-dimensional in vitro tumor models
Description:
The current research proposal intends to establish a technically elaborated screening routine for multidrug resistance reversing agents. This routine is based on the optical probe technique which allows to monitor fluorescent model substances for drug transport in the depth of tumor tissues. In parallel, reporter gene vectors of different drug resistance transporters based on green and fluorescent protein will be constructed. These reporter gene constructs will be expressed in different cancer cell lines (e.g. small cell lung cancer, prostate carcinoma, mammary carcinoma, hepatoma, melanoma, glioblastoma). After cultivation to multicellular tumor spheroids we will have a tool to analyze by the optical probe technique the expression of multidrug resistance transporters in living tissue. In parallel the optical probe technique allows by the use of fluorescent marker molecules to analyze drug transport kinetics in double fluorescence imaging routines.
Executive Institute:
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET) Details of BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments
Parent institution:
Federal Institute for Risk Assessment (BfR) Details of (BfR) (Berlin)
Contract period:
01. 11. 2004 - 31. 12. 2006
Participating Institutions:
- Institute of Physiology Details of Institute of Physiology
- Centre for Biomaterial Development Details of Centre for Biomaterial Development
- Department of Internal Medicine I, Cardiology, Angiology, Pneumology, Intensive Care Medicine (KIM I) Details of KIM I
- Bellvitge Biomedical Research Institute (IDIBELL) Details of IDIBELL
Funding Programme:
- BMELV / Core funding Details of BMELV / Core funding
- BfR Funding Details of BfR Funding
Subject:
- Food Chemistry
- Food Processing
- Biotechnology
- Food microbiology
Purpose of research:
Applied research
Funding Institutions:
- Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) Details of (BMELV)
- Federal Institute for Risk Assessment (BfR) Details of (BfR)
Project Management Agency:
- Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) Details of BMELV
- Federal Institute for Risk Assessment (BfR) Details of BfR