We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Developing a high-throughput genotype-independent regeneration system for accelerating breeding innovation in sugar beet (InnoBeet)
Project
Project code: 031B0556
Contract period: 01.06.2018
- 31.05.2020
Budget: 499,999 Euro
Purpose of research: Experimental development
Keywords: sugar beet, in-vitro regeneration, recalcitrance
Sugar beet is one of the most important industrial crops in Europe and Germany. Traditional breeding approaches gained significant yield increases as well as an improved tolerance against stress and pests. However, these challenging tasks are time-consuming and draw considerable resources, but can be accelerated through application of modern biotechnological methods. An essential requirement for those approaches is, however, an efficient in vitro regeneration system. Although this is applicable for sugar beet, it is very much genotype-dependent and thus associated with strong recalcitrance and low in vitro regeneration rates in many sugar beet genotypes. The aim of this project is to develop a universal protocol allowing the genotype-independent regeneration of sugar beet protoplasts. This will be achieved by establishing an innovative automated high-throughput microscopic procedure which will apply time-resolving image-analysis techniques to identify competent cells in regeneration-competent and recalcitrant genotypes. This will allow to quantify the proliferation rate of regeneration-competent and recalcitrant genotypes. The procedure will then be used to optimize the composition of proliferation media and to identify proliferation-modulating components. Transcriptome differences will be determined by transcript profiling in order to identify target genes which contribute to recalcitrance. Transcript levels of potential regulatory master genes will be altered in order to validate their involvement and to modulate the proliferation rates. These measures will allow defining efficient regeneration protocols which are genotype-independent and will enable to apply modern techniques of breeding research on sugar beet and to accelerate targeted breeding of new varieties with improved properties. In the long term, the knowledge gained through this project regarding reprogramming of plant-derived single cells will significantly facilitate optimizing the regeneration competence of other crops.
Section overview
Subjects
- Plant Breeding
- Biotechnology
- Process engineering
