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Imprint
Identification of genetic factors influencing replant disease in apple (OrdiAmur)
Project
Project code: JKI-ZGO-08-4209
Contract period: 01.01.2019
- 31.12.2021
Purpose of research: Applied research
The aim of the 2nd phase of Ordiamur-project is to elucidate the genetic mechanisms for susceptibility and tolerance of apple plants to the Apple Replant Disease (ARD). Replant disease is also referred to as soil fatigue or soil sickness, and describes the phenomenon that the soil after replanting of the same species loses its function as a growth medium. As a result, the newly planted plants show poor vegetative development and decreased yields. The dwarfing apple rootstocks used in modern apple cultivation are all susceptible to the apple replant disease (ARD). A sustainable and economically viable solution would be to use the ARD-tolerant rootstocks. Until date, only a few Malus genotypes have been described as tolerant to ARD and less in known about the genetic mechanisms underlying the tolerance of Malus genotypes to ARD. Within the project (1) the genetic map established in Phase I should be replenished and saturated by genotyping-by-sequencing (GBS) in order to create a high-resolution map for the mapping population M9 x M. × robusta 5. (2) Moreover, the phenotypic data of the mapping population from phase I should be ensured due to two further greenhouse experiments. The genetic and phenotypic data are the prerequisite for a subsequent QTL mapping for reduced susceptibility to ARD. (3) The third task is the further validation and characterization of selected candidate genes from phase I. For this, their expression in response to a targeted inoculation with selected isolates, which can cause ARD as well as after waterlogging will be estimated. Additionally, root samples of seedlings of the mapping population should be investigated. (4) Afterwards selected candidate genes should be integrated into the genetic map. (5) For the functional verification of the identified candidate genes, two silencing-systems will be established in apple. The first system is a virus-induced silencing method; the second system is to be based on CRISPR/Cas9.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Arboriculture
- Genetic Resources
Framework programme
Funding programme
Excutive institution
Institute for Breeding Research on Horticultural Crops (JKI-ZG)
Participating institutions
- Helmholtz Centre for Environmental Research (UFZ)
- Technical University of Brunswick (TU-BS)
- Technical University of Dortmund
- University of Bayreuth
- Leibniz University of Hannover
- Helmholtz Zentrum München, GmbH German Research Center for Environmental Health (GSF)
- Center for Business Management in Horticulture and Applied Research (ZBG)
- Landwirtschaftskammer Schleswig-Holstein (LWKSH)
- Hochschule Bonn-Rhein-Sieg (H-BRS)
