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Data Protection Declaration
Imprint
Development of a diagnostic system for detection of WNV infection and development of a genetic engineered vaccine against WNV infection.
Project
Project code: 2813200306
Contract period: 01.01.2007
- 31.12.2009
Budget: 1,283,465 Euro
Purpose of research: Experimental development
During the project a vaccine candidate was developed which is capable to fully protect mice from a West-Nile Virus (WNV) infection and forms the basis for further development in veterinary and human medicine. Major components of this vaccine are a DNA-plasmid and recombinant protein. Nucleic acids coding for structural proteins of WNV were inserted into specially engineered DNA plasmids. By doing so, a DNA vaccine was established which could be used successfully in protection studies. Functional nanoparticles were developed for the efficient delivery of DNA molecules into live cells. These particles consist of a cationic polymer and body-own proteins. They show no side-toxicity in vitro. In addition, micro-RNAs, small non-coding RNAs, were tested to serve as a biomarker for WNV infections. In a third part of the project, epidemiological studies were carried out to look for WNV-genomes in birds in Germany. To this end, cloacal swaps were collected and analyzed by methods for the detection of RNA. During the course of the project, no evidence for WNV circulation in Germany was obtained.
Section overview
Subjects
- Animal health
