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Imprint
Application of 2-DE-based proteomics for biomarker detection in human plasma
Project
Project code: BfR-CHS-02-G 1330-102
Contract period: 01.01.2004
- 31.12.2004
Purpose of research: Basic research
The application of methods of molecular toxicology is increasingly discussed also in the field of regulatory toxicology. A major issue is the question, wether these biochemical methods can be used to supplement conventional endpoints of health related toxicology. Mostly due to high costs, the use of methods of molececular toxicology (‘omics‘) is mainly restricted to pharmaceutical or industrial companies. In order to acquire expert knowledge in this field, research must also be performed in governmental institutions. At BfR, a joint research project was started in 2001 which evaluated the applicability of genomics and proteomics to detect early biomarkers for carcinogenicity using an accepted animal model. In 2003, a proteomic in vitro study was started. Another promising approach of ‘omics’ conssists in the analysis of ‘non-invasive’ biological samples, which allows processing of samples from human donors. This way, exposure and induction of health effects of chemical substances can be detected and urgently demanded human biomarkers may be discovered. As human plasma is an important material for non-invasive analyses, a project was started to establish techniques to effectively extract proteins from human plasma. After consolidation of methods, existing samples from TCDD-exposed humans will be analysed to evaluate the potential of 2-DE-based proteomics in this field.
Section overview
Subjects
- Biotechnology
- Toxicology
