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Development of a contact allergen-induced T-cell assay using Langerhans cells: 2009: Expression of lineage specific T-cell transcription factors as biological endpoint suitable to indicate chemical induction of sensitization 2010: Measurement and quantification of transcription factors via qRT-PCR and FACS
Project
Project code: BfR-PRS-08-1322-345-09
Contract period: 01.01.2009
- 31.12.2010
Purpose of research: Applied research
Culture systems of dendritic cells are currently in the focus regarding development of in vitro test methods for sensitizing compounds present in consumer products. Allergen-reactive cytotoxic T-cells are responsible for typical tissue damages arising in the process of contact dermatitis. In order to imitate such type IV allergic reactions in skin of experimental animals, in this project Langerhans cells (LCs) should be introduced in a new, animal-free T-cell test in vitro. The capacity of allergens to indirectly induce the expression of specific transcription factors in T-cells via stimulated (allergen-treated) LCs will be measured by real-time PCR. Here, the applicability of the transcription factors Runx3, T-bet, GATA-3, RORgammat and FOXP3 for indicating LC-mediated differentiation towards cytotoxic T cells, TH1, TH2, TH17 or TReg-cells, respectively, will be investigated for the first time in a system testing chemical allergens in vitro. In this project functional immune responses of effector T-cells will be quantified. Thus, the molecular responses of T-cells interacting with allergen-triggered LCs can be evaluated in light of the common type IV contact allergen classification according to Coombs and Gell. In addition, the possible contribution of regulatory T-cells and TH17 cells in pathogenesis of type IV allergy will be illuminated.
Section overview
Subjects
- Biotechnology
