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Characterization of the Solanum tuberosum proteome under conditions of nitrogen deficiency and drought stress as basis for breeding of nitrogen efficient and drought tolerant starch potatoes

Project

Climate change

This project contributes to the research aim 'Climate Change'. What are the sub-aims? Take a look:
Climate change


Project code: JKI-RS-08-3366
Contract period: 01.10.2012 - 31.05.2016
Purpose of research: Applied research

The intensified agricultural production of renewable raw materials bears the risk of environmental pollution by leaching of nitrogen and emission of greenhouse gases. Nitrogen availability, uptake efficiency and leaching are directly linked to the amount of water in the soil. Scenarios for climate change predict prolonged periods of drought in Central Europe in springtime and early summer, the time of maximum nitrogen uptake of cultivated potatoes. Comprehensive phenotyping of starch potato genotypes is intended in terms of nitrogen uptake and utilization efficiency under drought stress conditions with a special focus on modifications in the proteome. Within this subproject different genotypes will be characterized in response to drought and nitrogen deficiency stress in a rain-out-shelter. In addition, an in vitro screening for nitrogen efficiency will be performed and essential changes in the proteome under stress conditions will be determined. One aim is the identification of proteins that are specifically abundant in tolerant genotypes. Selected genotypes are grown under stress conditions in pot experiments. Morphological and biochemical parameters will be continuously evaluated and correlated with stress tolerance characteristics. Identified differentially reacting genotypes will be used for investigating alterations of the proteome in response to nitrogen deficiency stress. The aim of the project is the combined analysis of N-efficiency and drought tolerance features. The intensified production of renewable raw materials poses the risk of environmental pollution by leaching of nitrogen and emission of greenhouse gases. Nitrogen availability, uptake efficiency and leaching of N are directly linked to the amount of water in the soil. Scenarios for climate change predict prolonged periods of drought in Central Europe in spring and early summer, which is the time of maximum nitrogen uptake in potatoes. Therefore, the project aims at the comprehensive phenotyping of starch potato genotypes in respect to nitrogen deficiency and drought stress responses with a special focus on modifications in the shoot proteome. Within the subproject conducted at the JKI, 17 starch potato genotypes were characterized in 5-l-pots and 540-l-containers in rain-out-shelter experiments under control conditions, drought stress, nitrogen deficiency and a combination of both stresses. The determined morphological, physiological, biochemical and agronomic parameter allowed discrimination of tolerant and sensitive cultivars. In addition, the same set of genotypes was characterized for the response to N-deficiency under in vitro conditions. Genotype specific differences were observed particularly concerning root development and speed of nitrogen uptake. Cultivars reacting identical in all three experimental systems were identified. Proteomic studies were performed with two genotypes contrasting in N-deficiency response. Proteins were isolated from shoot tips of in vitro plants cultured for 11 days on media with 60 and 7.5 mMol/l N and separated by 2D IEF/SDS PAGE. The proteome patterns were significantly affected by N-deficiency and genotype dependent differences were observed. Out of 59 most differentially regulated proteins in the sensitive and 47 respective proteins in the tolerant genotype, 93 % were identified by MALDI TOF/TOF MS and attributed to nine functionally metabolic groups.

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Subjects

Framework programme

BMEL Frameworkprogramme 2008

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