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Imprint
Differentiation of the potato wart pathotypes by using newly detected DNA sequences
Project
Project code: JKI-EP-08-2212
Contract period: 01.07.2013
- 31.07.2016
Purpose of research: Applied research
In a FNR network project, after mass sequencing of potato sprouts infected with the quarantine fungus Synchytrium endobioticum, the causal agent of potato wart, those DNA sequences will be surveyed which do not show homology with the potato or other microorganisms, by sequence comparison within international data banks. Since there are only ITS sequences available for the detection of potato wart the newly generated DNA sequences are used for differentiating the pathotypes. This fast and reliable detection of the pathotypes is important, since in case of a new infestation case of potato wart only pathotype resistant potato varieties are allowed to grow in the vicinity of the infected field. The wart pathotypes will probably differ only slightly, a survey for point mutation for example will be the best approach since point mutations of potato wart pathotypes might easily be detected by restriction analysis.
Wireworms (Agriotes sp.) - whose imagines are the click beetles - are an increasing problem in agriculture. The morphological differentiation is difficult because the mouth tools (mandibles) of the wireworms are not always informative due to signs of wear. A PCR detection backed on sequenced mitochondrial DNA has been developed to ensure a proper differentiation of the species on the basis of single nucleotide polymorphism. Therefore, with the primers a distribution of the species in Germany can be determined, and one can react diagnostically properly when new wireworm populations appear. In 2016, the collected wireworm populations could be diagnosed with these primers and allocated to the proper varieties when a visual diagnosis was questionable.
Section overview
Subjects
- Plant Breeding
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
