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Introgression of scab and powdery mildew resistance from M. orientalis collection material in to apple breeding lines
Project
Project code: JKI-ZGO-08-4192
Contract period: 01.01.2017
- 31.12.2019
Purpose of research: Applied research
Keywords: resistenz breeding, Malus, scab and powdery mildew resistance
The project is aimed at introgression of scab and powdery mildew resistance originating from the wild apple species M. orientalis in apple breeding material. M. orientalis was gained from two collection trips to the Caucasus region. Apple were harvested and seeds released. Seeds were planted at ZO and inoculated with scab and powdery mildew. Five genotypes flowering in 2016 already and showing no incidence to scab and/or powdery mildew were crossed with Golden Delicious. Seeds will be planted in 2017 and inoculated with scab and powdery mildew to analyse the segregation of the traits and to get phenotypic data for mapping. According to the results one or two populations will be choosen for screening with a set of microsatellite markers. The set should cover all 17 linkage groups. Genotypic and phenotypic data will be used for mapping the resistances. An alternative approach is the establishment of resistant and susceptible bulks and the development of SNPs linked to the bulks. The sequences of linked SNPs will than be mapped to the Golden Delicious genome. Molecular markers will be establish for marker assisted selectoin. Additionally the loci will be compared to resistance genes already known to prove if new resistances are present in M. orientalis, which could be used to improve resistance breeding.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Arboriculture
Framework programme
Funding programme
Excutive institution
Institute for Breeding Research on Horticultural Crops (JKI-ZG)
