Collaborative project: Development of a systematic breeding strategy for hydrangea (Hydrangea macrophylla ssp. macrophylla) considering interploid crossings - Subproject 1 | FISA - Forschungsinformationssystem Agrar und Ernährung

Collaborative project: Development of a systematic breeding strategy for hydrangea (Hydrangea macrophylla ssp. macrophylla) considering interploid crossings - Subproject 1

Project

Production processes

This project contributes to the research aim 'Production processes'. Which funding institutions are active for this aim? What are the sub-aims? Take a look:
Production processes

Project code: 28RF40011
Contract period: 01.02.2015 - 28.02.2019
Budget: 183,543 Euro
Purpose of research: Experimental development
Keywords: horticulture, production of ornamentals

In hydrangea (Hydrangea macrophylla ssp. macrophylla), diploid as well as triploid cultivars exist, however, the ploidy level is not known for all varieties and genotypes, since first results on this topic have not been published until 2000 – 2007. Triploid varieties are often strikingly vital and healthy. Moreover, they are fertile and can be crossed with diploid genotypes. Notwithstanding, considerable crossing barriers do exist depending on the crossing combination. It is not known, why these barriers do exist, nor how they can be overcome. Hence, the aim of the project is to analyze the inheritance of the ploidy level in interploid crossings, to investigate whether aneuploids develop, and to test their fertility. From these results, a breeding strategy including diploid and triploid varieties will be deduced. Comprehensive cytogenetic analyses of interploid crossings in hydrangea will be performed. The ploidy level of the entire breeding material of the cooperation partner will be screened using flow cytometry and chromosome counting where required. In crossings with known fertility problems, the ploidy level of the parent plants, pollen viability, fertilization, seed development, and the ploidy level of the offspring will be monitored. Likewise, systematic interploid crossings will be undertaken including analyses of the fertilization process, the seed development, and the ploidy level as well as of the fertility of the offspring. The meiosis of triploids will be monitored in detail using cytogenetic chromosome staining. Depending on the results of these work packages, a breeding strategy including interploid crossings will be developed.

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