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Collaborative project: Identification of quantitative resistance for production of new cultivars with durable broad-spectrum resistance against Phoma lingam causing stem canker of rapeseed - subproject 10 (PhomaDur)

Project


Project code: 2818205915
Contract period: 10.08.2017 - 09.08.2020
Budget: 35,529 Euro
Purpose of research: Applied research

Leptosphaeria maculans (anamorph Phoma lingam) causes stem cancer of oilseed rape, the most important fungal disease of oilseed rape in Germany. In current rapeseed varieties qualitative monogenic race-specific resistance genes have been overcome by the evolution of the pathogen. This represents a new challenge to the genetic improvement of durable fungal resistance in modern cultivars. Thus, genomic regions and candidate genes involved in quantitative resistance against Phoma lingam will be identified by using an interconnected multi-parental mapping population. Quantitative resistance of winter oilseed rape against a broad spectrum of fungal races will be characterized using genetic and pathophysiological analyses. Molecular markers for application in marker-assisted selection for broad spectrum resistance against Phoma will be developed. The project consists of a phytopathological part, a breeding part and material development and field trials by the participating plant breeding companies. Within the phytopathological part possibly race-specific and broad-spectrum resistance mechanisms are investigated. The development of diagnostic markers for Phoma resistance is targeted within the breeding part by a combination of genetic and genome-based Next Generation Sequencing analysis and marker technologies. A genetic mapping approach is applied on a population including crosses from seven commercially parental lines with different quantitative resistance backgrounds. The analysis will also be completed by whole genome resequencing and optical mapping of the seven parental lines. Molecular markers will be derived and subsequently validated in a Brassica napus diversity set for their suitability for marker-assisted selection of rapeseed for resistance to a broad spectrum of Phoma races.

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Subjects

Associated projects: PhomaDur

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