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Optimizing the pain management in the mouse-osteotomy-model – Integration of a refinement study in a basic research study (RefineMOMo)
Project
Project code: BfR-ZEBET-08-1328-542
Contract period: 01.01.2016
- 31.12.2016
Purpose of research: Applied research
We plan to examine whether the non-invasive application of Tramadol or Buprenorphine via the drinking water provides a continuously, stress-free analgesic administration in the mouse-osteotomy-model and is therefore superior to the regular injection (including handling and restraint). In addition, to assess pain, wellbeing and severity, model-specific, clinical and behavioural parameters will be evaluated. Subsequently, we will be able to give evidence for the analgesic efficacy of Tramadol and Buprenorphine and possible adverse analgesia effects in the mouse-osteotomy-model. Different dosages of Tramadol according to the GV-SOLAS recommendations will be compared (0,1 mg/ml und 1 mg/ml) as well as Buprenorphine (0,009 mg/ml) as potent alternative. Moreover, we will assess whether the change of the analgesic or the medication itself has an effect on the fracture healing. In order to enhance the knowledge on refinement options in basic research studies and to reduce laboratory animal usage, we conceived a refinement study embedded in a basic research study in the mouse osteotomy model to show the possibility to combine both studies.
The application of analgesics in adequate dosages of Tramadol and Buprenorphine via the drinking water is suitable for pain management in the mouse-osteotomy-model. Within the 3 days post-OP, the animals take up continuously the treated drinking water. However, a potential “overdosing” of Tramadol has an influence on the wellbeing and condition of the animals without improvement of pain parameters. The application of analgesic via the drinking water should be combined with an adequate analgesia during the painful procedure (reduced water and food intake post-OP). The selection of parameters for pain assessment should be done carefully and address different assertions while working together with experts is beneficial. There were no significant differences in the fracture healing, but within a running study the pain management should only be adopted conditionally. The combination of a basic research study and a refinement study is expedient in order to effectively reduce the lab animal usage. Lang, A (2016) LAS, 50(6): 433-441
Section overview
Subjects
- Animal health
Framework programme
Funding programme
Excutive institution
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
