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Imprint
Establishment of recombinase vector system in apple to generate transgenic plants with increased resistence to fungal pathogens
Project
Project code: JKI-ZGO-08-4145
Contract period: 01.07.2007
- 30.06.2010
Purpose of research: Basic research
The project is aimed at the development of a site-specific Flp/FRT-recombinase system in apple (Malus domestica) to generate marker gene free transgenic plants. The usability of this alternative strategy for excision of selectable antibiotic resistance genes will be investigated and the efficiency of the system will be analysed by using a specific monitoring cassette. The project is focused on the exploitation of innovative strategies limiting the transferred gene sequences to a functional essential degree.
The Flp/FRT recombinase system will be used for the regeneration of transgenic apple plants with increased resistance to fungal pathogens like apple mildew (Podosphaera leucotricha) and apple scab (Venturia inaequalis). Resistance will be induced on the basis of a directed RNA interference system (RNAi) by silencing calss 5 chitinase synthase genes in P. leucotricha and V. inaequalis.
The project is part of the program 'Research and development of methods for safer applications in bio- and gene technology' , it is financed by the State of Saxony, Germany in the frame of the Biotechnology Program.
Section overview
Subjects
- Plant Breeding
- Arboriculture
Framework programme
Funding programme
Excutive institution
Institute for Breeding Research on Horticultural Crops (JKI-ZG)
