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Illumination of the transmission mechanism of soil-borne wheat mosaic virus by its vector, Polymyxa graminis and identification of novel resistance factors in barley
Project
Project code: JKI-EP-08-2313
Contract period: 01.10.2018
- 31.10.2021
Purpose of research: Applied research
Keywords: soil-borne viruses, soil-borne wheat mosaic virus, infection mechanisms,antiviral resistance
Polymyxa graminis-transmitted soil-borne viruses cause severe losses in cereals worldwide. As the viruses remain infectious inside polymyxa spores for decades, cereal cultivation on contaminated soil is only economic by growing resistant cultivars. Unfortunately, resistances to these viruses are readily overcome by the viruses, thus necessitating innovative resistance strategies. One way to identify novel resistance strategies is the illumination of the molecular mechanisms underlying the interaction between polymyxa-transmitted soil-borne viruses, thereby identifying candidates for virus control by the targeted modulation of host processes involved in infection. Therefore, the objective of the proposed work is the identification of key factors determining the P. graminis - Soil-borne wheat mosaic virus (SBWMV) - barley interaction. As virus transmission and movement are key processes influencing infection, the work specifically elucidates the function and interaction network of the SBWMV transmission factor CP-RT and the SBWMV movement protein (MP). The previewed work includes a high-resolution in vivo fluorescence microscopic analysis of the virus transmission process and virus movement between root cells. In parallel, we will identify and functionally characterize MP- and CP-RT-interacting host factors regarding a role in antiviral resistance. Candidate host factors interacting with SBWMV proteins will be identified in a protein biochemical approach and a role of the candidate host factors in virus infection will be determined with reverse genetic methods. The proposed work will significantly advance our understanding of the transmission and infection mechanisms of polymyxa-transmitted soil-borne viruses. By identifying novel resistance factors in the natural SBWMV host barley, the project will directly contribute to the development of sustainable solutions for agriculture by allowing direct inclusion of the identified resistance factors into resistance breeding programs.
Section overview
Subjects
- Plant Breeding
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
