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Collaborative project: Development and validation of new methods for the qualitative detection and quantification of fish, crustacea and molluscs besides of insects as potential food allergens. Subproject 1 (AQUALLERG-ID)
Project
Project code: BfR-LMS-08-1337-205, 281A103016
Contract period: 01.04.2019
- 31.03.2022
Budget: 329,850 Euro
Purpose of research: Applied research
Keywords: food analysis, food safety, labelling, product safety, allergens, allergy/ incompatibility response, food toxicology
The project aims to close analytical gaps in terms of species rich allergen groups such as fish, crustacea and molluscs. BfR part of the project: The aim of BfR’s project part is the development of a robust and reproducible screening-assay for the detection of species-specific DNA derived from aquatic organisms. For this, reliable real time PCR (polymerase chain reaction) methods will be employed in an efficient technical variation based on the principle of “Low Density-Arrays” using 384 micro-cavities. Hence substantially more species and/or groups of organisms can be tackled in one analytical step, circumventing frequently occuring specificity and sensitivity problems. The work program comprises the development of new real time PCR systems as well as the adaptation of published systems. The approach will end in a “ready-to-use” format. The operator only has to apply extracted DNA and mastermix into the small-sized cavities (~ 3-10 µl) of pre-prepared PCR plates. All reagents (primer/probes) needed will have to be spotted and stabilized by drying. Plate spotting will be done automatically to achieve a high degree of reproducibility. The assay encompasses species of regional meaning but also species which are rarely sold in Germany but do play an important role on the global markets and import. The choice of the species will be made on basis of a sound data base research. The selection of real time PCR systems, in addition, affords the adaptation to a unique temperature/time program. On the one hand, approaches will be developed using universal SybrGreen-Mastermixes without probes. This kind of screening decreases the costs for multiple analysis significantly. On the other hand, in parallel primer/probe-assays will be developed to meet the requirements of standard methods to include a confirmation reaction. The assays shall be published and made available to any laboratory without restrictions. Furthermore, in close cooperation with the company IMGM GmbH (Martinsried, Bavaria, Germany), novel approaches will be developed based on NGS (Next Generation Sequencing) technology, enabling the multiple sequencing of species specific DNA by metabarcoding.
Section overview
Subjects
- Marine fisheries
- Physiology of Nutrition
- Biotechnology
- Toxicology
Framework programme
Funding programme
Excutive institution
Funding institution
Project management agencies
Associated projects: AQUALLERG-ID
- Collaborative project: Development and validation of new methods for the qualitative detection and quantification of fish, crustacea and molluscs besides of insects as potential food allergens. Subproject 2
- Collaborative project: Development and validation of new methods for the qualitative detection and quantification of fish, crustacea and molluscs besides of insects as potential food allergens. Subproject
