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Imprint
Simultaneous determination of hemoglobin adducts of heat-induced contaminants in food with a modified Edman degradation
Project
Project code: BfR-LMS-08-1322-690
Contract period: 01.01.2018
- 31.12.2019
Purpose of research: Applied research
The presence of genotoxic substances in food in the form of process-related contaminants makes targeted regulatory measures difficult. Because of the genotoxic and carcinogenic effects, it is often not possible to set a limit for the daily intake. Still, it is important to clarify if the daily dietary exposure to these substances poses a risk to humans. In this context, the difficult estimation of exposure is problematic because levels in industrially produced foods vary widely. Also, many contaminants are formed in the heat treatment during the preparation of the meals in the kitchen of consumers. One solution would be to determine biomarkers of internal exposure, e.g. reaction products of the reactive metabolites. For this purpose adducts at amino acid side chains of proteins, in particular the blood proteins serum albumin (SA) and hemoglobin (Hb), are well suited. These are particularly good molecular dosimeters since they are available in relatively large quantities. In addition, the lifetime of the proteins is clearly defined and active repair systems for adducts do not exist. The most commonly used method in protein adduct analysis is the Edman degradation, in which the modified valine is cleaved off at the N-terminus of hemoglobin. Analytical methods have already been published in the FG54 for quantifying the hemoglobin adducts of the heat-related contaminants furfuryl alcohol and glycidol. Furthermore, work is underway to analyze the adducts of acrylamide and glycidamide as well as of methylglyoxal at the N-terminus of hemoglobin. The aim of this project is to develop an analytical method for the mass spectrometric determination of all five biomarkers of internal exposure. It should be noted that there is already a similar method by M. Törnqvist (Carlsson, H. (2017) Chem. Res. Toixcol. 30, 1157). In this, several adducts are quantified by UPLC-MS/MS using only a single deuterated substance as an internal standard. From an analytical point of view, this method is considered inaccurate. We aim at a method in which ALL adducts are quantified using specific isotope-labeled substances. This is more complicated; but the results are more resilient. In particular, two steps of the analysis have to be optimized for the development of the technique in such a way that they
Section overview
Subjects
- Biotechnology
- Toxicology
