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Collaborative project: Development of innovative detection methods for potato wart disease as a basis for ensuring potato production in Germany - subproject 1 (INNOKA)
Project
Project code: 2819112519
Contract period: 09.09.2019
- 08.11.2022
Budget: 777,057 Euro
Purpose of research: Experimental development
Keywords: plant protection, potato wart diesease, fungus sorus, Synchytrium endobioticum
Potato wart disease is one of the most important quarantine pests effecting potato production. This disease is caused by the obligate biotrophic, soil-borne fungus Synchytrium endobioticum. Potato wart disease locally occurs in nearly all European countries. In the past 50 years, about 1,500 infection foci with an area of 650 ha were registered in Germany. Former infections occured mainly in domestic gardens, but increasingly also agricultural areas are affected. Especially in areas with extensive potato production, like Emsland, the situation is getting more and more problematic. The current detection of spores indicates that the causal agent of this disease has spread unseen. The actual distribution of Synchytrium endobioticum in Germany is unknown. The present project aims for closing this knowledge gap. The overall goal of the project is to develop innovative methods for detection of S. endobioticum to minimize the spread of potato wart disease in Germany and to ensure potato production and export for the future. Therefore, effective methods for sampling and sample preparation will be developed, as well as molecular methods for identification and characterization of resting spores. An important aspect is the identification of different pathotypes by molecular markers. Experiments with partial resistant cultivars should indicate, if they are suitable for cultivation in infection-prone areas. The activities planned for the recent project can be mainly divided into three parts. (1) Development of effective procedures for sampling and sample preparation for different types of soils. Therefor, the parameter for an efficient and cost effective strategy should be evaluated. The isolated spores should be analyzed microscopically and differentiated to others spores with visual similarities. (2) Molecular analyses are planned to identify different pathotypes based on molecular markers. Additional analyses to determine the genetic diversity of spore samples will be carried out. An important point is the optimization of extraction methods and determination of detection thresholds of all used methods. In addition, a molecular method for determination of viability of spores should be developed. (3) Different types of biotests should be carried out to determine their suitability for evaluation of viability of spores, as well the identification of infection thresholds.
Section overview
Subjects
- Crop Production
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Plant Protection in Field Crops and Grassland (JKI-A)
