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Rapid identification of psychrophilic pseudomonads from raw milk using MALDI-TOF MS (PseudoID)
Project
Project code: MRI-MBT-08-1040-pseudo-id
Contract period: 01.03.2021
- 01.02.2022
Purpose of research: Experimental development
The presence of psychrotolerant microorganisms and their heat-stable enzymes in raw milk can cause bitterness, increased viscosity and gelation in milk and dairy products, leading to product spoilage and associated significant economic losses in commercial milk production and processing. This is caused to a considerable extent by heat-resistant bacterial peptidases, which are mainly produced by bacteria of the genus Pseudomonas. They show a very high heat resistance and are only insufficiently inactivated by UHT treatment. Therefore, the rapid and reliable identification of these bacteria in milk and dairy products and the tracing of their sources on the farm and in processing plants is of great importance. Using the mass spectrometric technique MALDI-TOF-MS spoilage bacteria of the genus Pseudomonas, which are isolated from raw milk, are to be identified by their protein fingerprint. For this purpose, a database with reference spectra of Pseudomonas type strains has already been established. The aim of the project is to extend and complete the existing database with raw milk relevant Pseudomonas isolates to ensure the identification of product relevant strains. Therefore, raw milk isolates from defined farms will be collected and analysed by MALDI-TOF-MS. If the mass spectra of the isolates deviate significantly from the reference spectra of the type strains, the isolates are taxonomically identified using genome-based methods to complete the database. By this, the diversity of raw milk associated pseudomonads on species level will be analysed and possible farm specific "lead bacteria" identified. Furthermore, the spoilage potential of the raw milk isolates will be characterized by the determination of the proteolytic activity and the genetic organization of the relevant operon aprX-lipA2 and possible biomarkers will be identified using MALDI-TOF MS.
Section overview
Subjects
- Food Processing
- Food microbiology
Framework programme
Funding programme
Excutive institution
MRI - Department of Microbiology and Biotechnology (MRI-MBT)
