We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Implementaion of genomic selection in barley breeding
Project
Project code: JKI-RS-08-1134
Contract period: 01.09.2011
- 28.02.2015
Purpose of research: Experimental development
The project aims at establishing genomic selection in barley (in-breeding species). To achieve this, a large number of barley genotypes has to be genotyped (9K iSelect chip) and analysed in a first step in field and growth chamber experiments. Besides yield, special emphasis will be given with respect to phenotyping to drought tolerance and nitrogen uptake. Based on the data generated within BARSELECT first information on the applicability of genomic selection in inbreeders will be gained as well as broad information on the variation concerning drought tolerance and N-uptake in barley. Thereby, the project contributes to a directed improvement of winter barley and its adaptation to changing growing conditiond due to climate change.
Barley (Hordeum vulgare) is next to wheat the most important crop in Germany. The project aimed at establishing genomic selection as a new tool in barley breeding (in-breeding species). To achieve this, a large number of barley genotypes was genotyped via the Illumina 9K iSELECT chip, and phenotyped in field and growth chamber experiments. Besides yield, special emphasis was given to drought tolerance and nitrogen uptake. Based on the data generated within BARSELECT first information on the applicability of genomic selection in inbreeders will be gained as well as broad information on the variation concerning drought tolerance and N-uptake in barley. Thereby, the project contributes to a directed improvement of winter barley and its adaptation to changing growing conditions due to climate change. The JKI was mainly involved in phenotyping of the calibration set (780 genotypes) in 2-years field trials in years 2012 and 2013 and of the validation set (750 genotypes) in 2014 for agronomic important traits at Quedlinburg. For all years genotypic differences were observed for agronomic important traits, e.g. yield, thousand grain weight, and height. Concerning resistance to different pathogens genotypic differences were observed only for the calibration set since in 2014 no infection took place due to the weather conditions. In addition, the JKI developed a high-throughput screening system for drought tolerance on the basis of six physiological traits (chlorophyll fluorescence, relative water content, membrane stability, osmotic adjustment, content of soluble sugars and proline). Based on correlations to yield data obtained in rainout shelter trials for drought tolerance three of these traits (chlorophyll fluorescence, relative water content, osmotic adjustment) were selected for the analyses of the calibration and validation set. For these physiological traits a significant influence of the in vitro-drought stress as well as significant differences between the genotypes after the drought stress treatment were observed. Therefore, at the JKI a high number on phenotypic data was generated which enable a valid estimation of genomic estimated breeding values (GEBVs) of agronomic important as well as physiological, drought-relevant traits and the correlation of these GEBVs to real breeding values.
Section overview
Subjects
- Plant Breeding
- Climate Change
Framework programme
Funding programme
Excutive institution
Institute of Resistance Research and Stress Tolerance (JKI-RS)
