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Cytochrom P450 (CYP 2E1)-dependent metabolism in genetically modified cell lines
Project
Project code: BfR-CHS-08-1322-236
Contract period: 01.04.2008
- 31.12.2008
Purpose of research: Basic research
Acrylamide has been shown to be a carcinogen in laboratory animals in long term studies. The mechanism of tumor induction is presently not fully understood and it is assumed, that the metabolism is of special relevance. In the liver of mammals Acrylamide is metabolized by CYP2E1 to Glycidamide, which is proposed to be of relevance for the cancerogenic and DNA damaging effects. In this project the relevance of this pathway in humans will be investigated. With the aid of genetically modified cell lines it will be studied, if species differences in the CYP 2E1 dependent metabolism of Acryamide exist. Simultaneously the DNA damaging effects of Acrylamide and Glycidamide will be investigated in these cell lines.
Acrylamide is probably also a human carcinogen. The relevant metabolite with an initiating potential is glycidamide. This metabolite is generated from AA by a CYP2E1 dependent reaction. With regard to risk assessment the evaluation of the formation rate in different species is of importance. It was the aim to investigate this rate in genetically modified cell lines, expressing CYP2E1 from different species.
Section overview
Subjects
- Biotechnology