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Development of a nursery-infested flying brand management for the breeding of resistant spring barley under natural infestation conditions

Project


Project code: 2806OE028
Contract period: 01.04.2007 - 31.12.2009
Budget: 38,815 Euro
Purpose of research: Applied research

Spreading of loose smut (Ustilago nuda (Jens.) Rostr.) was observed during three vegetation periods in a breeding area under organic farming with segregating generations of spring barley up to F9 including lines with resistant parents and extended with susceptible varieties and genetic resources. Seed stripes with loose smut infection were implemented for a better spreading of the disease in some parts, which was proofed to be suitable. Plots with non-infected seeds of a susceptible variety were implemented in between the segregating lines to detect degrees of spreading. Selected lines without infection were inoculated with a loose smut suspension into the flowers of single ears starting in F5. From F5 to F7 most of the susceptible lines were identified by natural infection. One generation under natural infection was not enough to get a sustainable result about susceptibility, but only one out of ten inoculated ears gave additional information. For this reason it was suggested only to use seed stripes for disease spreading during three generations from F4 to F6 and artificial inoculation only for a check of resistance in F7 and for new accessions to test. With this method different origins of resistance can be handled parallel and it is possible to develop quantitative resistance on a low budget scheme aditionally.

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