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Imprint
Application of 2-DE-based proteomics in vitro
Project
Project code: BfR-CHS-02-L 814-02
Contract period: 01.05.2004
- 30.11.2004
Purpose of research: Basic research
The application of methods of molecular toxicology is increasingly discussed also in the field of regulatory toxicology. A major issue is the question, wether these biochemical methods can be used to supplement conventional endpoints of health related toxicology. Mostly due to high costs, the use of methods of molececular toxicology (‘omics‘) is mainly restricted to pharmaceutical or industrial companies. In order to acquire expert knowledge in this field, re-search must also be performed in governmental institutions. At BfR, a joint research project was started in 2001 which evaluated the applicability of genomics and proteomics to detect early biomarkers for carcinogenicity using an accepted animal model. In order to improve the potential of in vitro methods, ‘omics‘ methods are also evaluated in this field. In this study, 2-D electrophoresis-based proteomics is used to detect chemically induced changes in cell cultures. In a first step, robust techniques have to be established to successively extract and separate proteins from cell cultures.
Section overview
Subjects
- Biotechnology
- Toxicology
