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Data Protection Declaration
Imprint
Hepatotoxic combination effects of multiple residues of pesticides in vivo und in vitro
Project
Project code: BfR-SiP-08-1322-499
Contract period: 01.02.2012
- 30.11.2015
Purpose of research: Basic research
The evaluation of potential combination effects of multiple residues of pesticides is required according to Regulation EU 1107/2009. Since substances are evaluated for toxicological properties individually rather than in combination, science based risk assessment of mixtures is difficult. Additionally there is an intense discussion on potential effects in the low dose range, which is currently not tested. Consequently experimental analysis is needed to show if toxicologically derived threshold levels like the ADI are safe under the conditions of a cumulative exposure. Hence, an urgent need for research is recognized to analyze potential combination effects of pesticides in a broad dose range. To contribute to this topic an 28-d feeding study in rats has been conducted administrating azol fungicides individually and in combination. Besides this in vivo experiment also ex vivo and in vitro methods have been used to analyse possible combination effects of these fungicides. First results point at combination effects in the target organ liver at dose levels where also individual substances caused effects. Additionally arrays-based analysis has revealed evidence for signal transduction pathways involved in triazole mediated toxicity and marker genes. These results shall be confirmed within the last part of this project.
There is growing public concern that multiple residues of pesticides in or on food might cause combination effects on consumer health. Since substances are evaluated for toxico-logical properties individually rather than in combination, science based risk assessment of mixtures is difficult. Additionally there is an intense discussion on potential effects in the low dose range, which is currently not tested. Consequently experimental analysis is needed to show if toxicologically derived threshold levels like the ADI are safe under the conditions of a cumulative exposure. Hence, an urgent need for research is recognized to analyze potential combination effects of pesticides in a broad dose range. To contribute to this topic an 28-d feeding study in rats has been conducted administrating azol fungicides individually and in combination. Besides this in vivo experiment also ex vivo and in vitro methods have been used to analyse possible combination effects of these fungicides. First results point at combination effects in the target organ liver at dose levels where also individual substances caused effects. Additionally arrays-based analysis has revealed evidence for signal transduction pathways involved in triazole mediated toxicity and marker genes. These results shall be confirmed within the last part of this project.
Section overview
Subjects
- Toxicology
