Collaborative project: Biotechnological based breeding strategy to strengthen the resistance of barley against Ramularia - Subproject 2 | FISA - Forschungsinformationssystem Agrar und Ernährung

Collaborative project: Biotechnological based breeding strategy to strengthen the resistance of barley against Ramularia - Subproject 2

Project

Project code: 2814302807
Contract period: 01.04.2008 - 15.09.2011
Budget: 130,434 Euro
Purpose of research: Experimental development

In a cooperation of five barley breeding companies and three research groups conditions were to be set out for the breeding of barley with improved resistance to the fungal pathogen of Ramularia collo-cygni (RCC). A whole-plant test, a leaf-segment test, and a RealTime- PCR protocol for RCC-DNA detection were established to quantify infestation of plants with RCC. A screening of 40 spring barley cultivars and breeding lines gave a clear differentiation of entries with either high or moderate to low susceptibility to RCC. An association study involving 78 molecular markers (SSR, STS, SNP) revealed significant associations of markers and RCC susceptibility on all the seven barley chromosomes. In a QTL study, a DH population was phenotyped over three years at two locations. From these studies, a QTL located on the short arm of chromosome 4H was identified which appears well-suited for marker-assisted selection of breeding lines with decreased susceptibility to RCC in breeding programmes.

show more show less

Section overview