Logo of the Information System for Agriculture and Food Research

Information System for Agriculture and Food Research

Information platform of the Federal and State Governments

Collaborative project: Phenomics, transcriptomics and genomics - an integrated approach to enhance selection for drought tolerance in barley - subproject 4

Project


Project code: 2814506510
Contract period: 01.10.2010 - 30.09.2013
Budget: 255,504 Euro
Purpose of research: Experimental development

The drought stress responses of spring barley varieties were analyzed by a comprehensive gene expression study (RNA-seq) in subproject 3 (LfL). The results were the basis for the selection of 107 candidate genes which were resequenced in a set of spring and winter barley varieties in order to detect different haplotypes. Molecular markers for those candidate genes were developed and integrated into genetic maps. A genome wide association study (TP4) showed that 16 markers for candidate genes were associated with agronomic traits the rainout-shelter experiment (p>0.01). Expression analysis of a subset of 76 candidate genes in winter barley grown in the rainout-shelter and spring barley grown in the climate chambers lead to similar results. The results obtained in the project give valuable information about candidate genes and pathways connected to agronomic and physiologic traits under drought. All genetic markers for drought stress response developed at LfL can be used for innovative breeding strategies as well as for further scientific research.

show more show less

Subjects

Advanced Search