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Development of a Variable-Number-Tandem-Repeat Analysis (MLVA) typing system for Salmonella Enteritidis isolates
Project
Project code: BfR-BIOS-08-1322-204
Contract period: 01.01.2007
- 31.12.2007
Purpose of research: Applied research
The Multiple-Locus-Variable-Number-Tandem-repeat Analysis (MLVA) became within the last years an important tool to subtype various bacteria species including Salmonella enterica serovars. In the last year the MLVA method was established for Salmonella Typhimurium at the NRL-Salm. The fragment analysis of the PCR products is performed by an ABI 310 Sequence Detection System. Currently, there is no MLVA method for Salmonella Enteritidis available. In this project, such a method will be developed especially for S. Enteritidis. S. Enteritidis is with 67% the most prevalent serovar isolated from humans in Germany. Outbreak studies are therefore performed very often at the NRL-Salm in respect to this serovar. These studies are labour intensive and time consuming because of the high clonality of S. Enteritidis (specially PT4). MLVA offers a possibility to perform molecular subtyping until the strain level and consequently to understand epidemiological relationships of the strains tested better in comparison with other molecular methods, such as plasmid profiling, ribotyping or PFGE.
Section overview
Subjects
- Physiology of Nutrition
- Food microbiology
