We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Development, improvement and assessment of PCR methods for detection and subtyping of Yersinia
Project
Project code: BfR-BIOS-08-1322-217
Contract period: 01.02.2007
- 31.12.2008
Purpose of research: Applied research
PCR techniques for the subtyping of pathogenic bacteria, like e.g. MLST, AFLP, MLVA, have a high differentiation power. We want to apply MLVA and AFLP techniques for strains of the genus Yersinia harboring a type IV secretion system. Additionally, we will characterize Y. enterocolitica strains of the pathogenic bioserotypes. The aim is to develop and optimize these techniques for the use in the Consiliary Laboratory on Yersinia.We are investigating a conjugative system of Yersinia enterocolitica with close relationship to type IV secretion systems of other pathogenic gramnegative bacteria to elucidate if antimicrobial resistance or virulence genes are associated and transmitted by the transfer system. For the investigation Yersinia strains from different sources were used. These include clinical strains, food strains and strains from ani-mals (mostly porcine strains). Serological and biochemical typing revealed that the type IV harbouring Yersinia strains belong to Y. enterocolitica biotype 1A, Y. kris-tensenii and Y. frederiksenii. In cooperation with the VLA Weybridge, UK, we are studying now potentially pathogenic Y. enterocolitica biotype 1A strains which were isolated from diarrhoe patients. Typing methods like PFGE revealed that the strains are very heterogenous in comparison to strains belonging to the pathogenic bioserotype strains. So far, in all strains the type IV system is plasmid encoded.
Section overview
Subjects
- Food microbiology
- Toxicology
