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Quantitative and qualitative detection of foodborne pathogens using real-time PCR and typing of the pathogens with DNA microarray
Project
Project code: BfR-BIOS-08-1322-261
Contract period: 01.02.2007
- 31.12.2008
Purpose of research: Applied research
To minimise the risk of an infection for the consumer by contaminated food microbial quality control measurements by legislative authorities, in the food production and veterinary medicine play a major role. To fulfil the requirements legislative authorities and other diagnostic laboratories need reliable, fast and international accepted detection systems. Currently, the detection is mainly performed by traditional culturing of the microorganisms in artificial broth media followed by biochemical and serological identification needing several days until weeks. An alternative for the detection of food-borne pathogens is the polymerase chain reaction (PCR). Rapidity, specificity, sensitivity and automation of this technique are the most important characteristics of the PCR.Often, the characteristic properties of an isolate play a role for the outbreak investigation and other epidemiological questions. These properties can be reliably analysed by molecular methods nowadays.In frame of this continuing research project a developed DNA microarray for the characterisation and typing of Salmonella shall be optimised and validated. Selected Salmonella strains will be tested and its risk potential determined.
Section overview
Subjects
- Food microbiology
