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Data Protection Declaration
Imprint
Proteomic investigations of carcinogenic effects in primary rat hepatocytes: prevalidation of an in vitro hepatic model for detection of non-genotoxic carcinogens
Project
Project code: BfR-LMS-08-1322-416
Contract period: 01.04.2009
- 31.12.2011
Purpose of research: Applied research
Toxicogenomic approaches represent a potential tool for predictive toxicology and for gathering information about the involved mechanism of action of toxicants. At present this tech-nology is mostly used in vivo in order to obtain information on toxicity mechanisms from short-term studies for the risk assessment of substances. With regard to the ethical problems as well as the high requirement of time and costs of animal experiments, the development and validation of in vitro systems suited for carcinogen detection is urgently required. While it has been shown recently that discrimination between genotoxic and non-genotoxic substances is possible in vivo, until now there is no adequate hepatic in vitro test system available for detecting non-genotoxic carcinogens. In cooperation with the lab of Prof.Hengstler (IfADo) we established an in vitro system using gene expression data in response to treatment with the non-genotoxic carcinogen methapyrilene (see project 1334-177). The aim of this project is to assess the applicability of a primary rat hepatocyte in vitro system for the identification of biomarkers for non-genotoxic carcinogens via a complementary proteomic approach.
Section overview
Subjects
- Physiology of Nutrition
- Toxicology
