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Refinement of the LCSA (loose-fit coculture-based sensitization assay)
Project
Project code: BfR-ZEBET-08-1328-202
Contract period: 01.08.2007
- 31.07.2008
Purpose of research: Applied research
We are refining a new animal-free assay for determination of the sensitizing potential of a substance. The design of this assay is based on current immunological knowledge of pathogenesis of allergic contact dermatitis. It integrates human dendritic cells and keratinocytes, which are both known to be critically involved in-vivo. The read-out system uses molecular responses typically occurring after encounter of the skin with contact allergens. The assay provides concentration-response information. Thereby, the relative ability of a chemical to induce sensitization can be predicted. Additionally, the assay defines the border-concentration of general toxicity of a substance. We have called the assay LCSA, loose-fit coculture-based sensitization assay.In the funded project, we analyze whether the new assay detects the allergenic potential of isoeugenol. Isoeugenol is a weak allergen, and furthermore, it is a prohapten. As such, it must be metabolized to a molecule that can combine with a protein. In addition, we have established a cooperation with Professor Monika Schäfer-Korting, Pharmazie, FU Berlin. We are trying to combine the LCSA with an in-vitro assay for absorption and skin penetration.
Section overview
Subjects
- Biotechnology
Framework programme
Funding programme
Excutive institution
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
