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Imprint
Characterization and impact of yam bean mosaic virus
Project
Project code: JKI-EP-08-2203
Contract period: 01.03.2012
- 28.02.2014
Purpose of research: Basic research
Towards the end 2010, viral desease symptoms were observed in a yam bean field in San Ramom, Junin, Peru. Small interfering RNA (siRNA) sequencing and assembly was performed on a sample showing severe mosaic and leaf deformation, and the full genome sequence of a virus could be assembled. Based on molecular analysis (amino-acid and nucleotide sequence identity) it was confirmed to be a potyvirus, however, clearly distinct to several bean viruses including bean common mosaic virus to whitch it is most closely related. This virus was designated as Yam-bean mosaic virus-isolate SR (YBMV-SR). The sequence of YBMV was highly similar to a partial potyvirus sequence (accession number AB289438) reported from yam bean in Indonesia. No cheap and rapid detection methods to identify YBMV-SR exist nor is it known wether the virus is seed-transmitted. Furthermore there is little or no information on the impact of this virus on yam bean production, its variability and occurence in the world, its alternative hosts, and on resistance among cultivated yam bean germplasm. The proposed study aims at answering some of these questions through development of novel and innovative detection methods (PCR and LAMP), molecular characterization of YBMV strains from Peru and other countries, the determination of experimental and wild host species as well as the study of virus ecology, natural distribution and variation. The project is complemented by a field trial to investigate the impact.
Section overview
Subjects
- Crop Protection
Framework programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
