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Diagnosis of virus diseases within certificatioin of pome fruit and development of strategies for maintaining the health status of certified basic material and rootstocks (DIAG-VER)
Project
Project code: 2812NA019, JKI-OW-08-1174
Contract period: 01.10.2012
- 31.12.2016
Budget: 397,255 Euro
Purpose of research: Basic research
The four in Germany still existing tree fruit basic material propation gardens and the certified rootstock producing nurseries will be supported to have the ability for producing 'vf' certified material. All scientific goals of the project on investigation of the apple viruses ASPV, ASGV, ACLSV and the unknown rubbery wood pathogen derive from the recommendations of the BMELV status workshop from 17. Nov. 2009 as well as the related BMELV feasibility study compiled by Dr. Beyme. The project focuses on improved knowledge of relevant viruses and virus testing within the filiation system for certified propagation material and control of virus status after thermotherapy. Transmission trials of the rubbery wood pathogen using Cuscuta species, rolling circle amplification detection trials, characterization of identified viruses and development of PCR for a rubbery wood pathogen will be done. Full length PCR's for ACLSV isolates and production of infecfious cDNA clones for ACLSV, ASPV and ASGV, followed by infection experiments on apple, pear and quince for the study of related diseases will be investigated. All results will be published in journals and communicated with the round table members 'Reiserschnittgärten' including the plant protection experts and nurserymen during the annual meeting of the working group 'fruit tree propagation gardens and certification of pathogen tested fruit crops'.
Double stranded RNA (dsRNA) from three apple trees with mixed infection with latent apple viruses (ASPV, ASGV, ACLSV) as well as rubbery wood and/or flat limb disease symptoms was isolated and sequenced using Illumina HiSeq2000 device. Sequence analysis showed low percentage of sequence reads (1-2%) which matched apple viruses and in most cases the coverage of the whole genome was not complete. De novo sequence assembly produced Contigs which corresponded to the lengths of complete ASPV, ASGV or ACLSV genomes. Phylogenetic analysis of assembled Contigs revealed new strains of all three viruses. Cross sections of the rubbery wood samples were analyzed with FT-IR spectroscopy and structural differences between healthy and diseased wood were found.
Section overview
Subjects
- Crop Protection
- Arboriculture
Framework programme
Federal Organic Farming Scheme and other forms of sustainable agriculture (BÖLN)
Funding programme
Excutive institution
Institute for Plant Protection in Fruit Crops and Viticulture - Fruit Cops (JKI-OW)
