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Breeding and cultivation of Circassian Dandelion (Taraxacum koksaghyz) - From wild plant to renewable industrial raw material. Subproject 3: Genetic analysis and marker-assisted selection sub-project 3: (TAKOWIND)

Project


Project code: 22002312, JKI-ZL-08-3181
Contract period: 01.07.2013 - 30.06.2016
Budget: 121,832 Euro
Purpose of research: Applied research

Russian dandelion (Taraxacum kok-saghyz) shall be developed via plant breeding to serve a sustainable resource of caoutchouc milk and inulin as raw materials for industrial purposes. Dandelion accessions shall be selected by use of efficient breeding methods, such as marker-assisted breeding, use of biomarkers, in vitro propagation, NMR analytics. Furthermore, seed of selected breeding lines shall be propagated and roots be harvested and assessed for caoutchouc milk and inulin contents. Extracts shall be processed at a pilot scale and evaluated in their physico-chemical properties.Work Schedule Segregating T. kok-saghyz populations will be analysed to reveal the inheritance of agronomic and metabolic traits. Isoprenoids and inulin will be quantified via NMR and plants with high contents will be selected for propagation. Molecular markers will be developed on the basis of a candidate-gene approach. Genome-wide markers will be used for species differentiation and marker-assisted background selection following interspecific hybridisation of Taraxacum species. Seed and root production of promising breeding lines will be performed on farmers fields.

As part of the joint project TAKOWIND, several genetic tools such as genetic maps of Russian dandelion and other molecular marker information were provided for breeding purposes. The genetic maps consist of 13 19 SNP markers spread over 8 linkage groups (corresponding to 8 chromosomes). These genetic maps provide the basis for mapping quantitative traits such as rubber content. The insights gained in this project contribute to establishing efficient breeding programmes, thus opening up a resource of natural rubber for a sustainable bioeconomy.

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Subjects

Associated projects: TAKOWIND

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