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Imprint
Molecular Charcterization of the resistance locus Ren3 against Erysiphe necator (powdery mildew) from the grapevine cultivar 'Regent'
Project
Project code: JKI-ZR-08-5174
Contract period: 01.08.2013
- 30.11.2016
Purpose of research: Applied research
The aim of this project is to unravel the mechanism of pathogen resistance encoded in the major QTL locus Ren3 on chromosome 15 of grapevine cultivar `Regent´.
To analyze this locus, genomic sequences partially covering the QTL region will be assembled. Resulting contigs have to be verified by PCR techniques. Remaining gaps will be seeked to be closed by 'long range' PCR. Bioinformatics analysis (identification of protein coding sequences, search of related genes with known functions in data bases) will identify positional candidate genes of resistance.
These candidate genes will be investigated for their functional relevance. Gene expression analyses will be performed in resistant and compared to susceptible grapevines in relation to microscopic observations on the host/pathogen interaction in the early stages of plant defense.
Diversity studies of the candidate gene sequences in a comprehensive set of resistant and susceptible grapevines will reveal sequence variation and single nucleotide polymorphisms (SNPs). These need to be correlated to the expression of resistance by QTL analysis and association genetic approaches. In this way further indications on their functional relevance will be obtained.
A hypothetic model of the successful defense operating in `Regent´ has to be developed from the data. Candidate genes with significant roles in this pathway need to be transferred into susceptible grapevines by genetic transformation. Transgenic plants will be tested for newly acquired resistance traits to validate the genes functions.
This project and the acquisition of resistance mechanisms will provide fundamental knowledge for better informed use of resistance loci in approaches to pyramide several of them for sustainable resistance. In addition, tightly linked molecular markers to the resistance trait will become available and appicable in marker assisted breeding in high throughput assays.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Viticulture
