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Characterisation of the interaction between L. monocytogenes isolates and the microbial raw-milk community by metatranscriptomic analysis
Project
Project code: BfR-BIOS-08-1329-466
Contract period: 01.01.2012
- 31.12.2012
Purpose of research: Basic research
Raw-milk from cows is the starting product of a great variety of dairy products, from which 60 kg milk and 21 kg of cheese are consumed by each German each year. Listeria monocytogenes-contaminated products are known to cause Listeriosis, a severe infection with high mortality for risk persons such as pregnant, elderly or children. Raw milk is accompanied by a diverse microbial community, which is in general able to metabolize all milk compounds. A part of this community such as Lactobacillus lactis are added in industrial scale to milk products as probiotic microorganisms.
The aim of this project is to investigate interaction patterns between the raw-milk community and L. monocytogenes and whether such interactions alter the metabolic productivity of the entire community. To undergo this project extensive high-troughput sequencing on transcriptomic level of raw milk community after addition of selected L. monocytogenes isolates are planned. As result sequence information and sequence abundance of expressed genes will be revealed.
Section overview
Subjects
- Animal Husbandry
- Toxicology