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Collaboratvie project: Identification of molecular marker for soil borne virus resistance in wheat - subproject 2 (ReBoVi)

Project


Project code: 2814601913, JKI-RS-08-3382
Contract period: 09.09.2014 - 31.05.2018
Budget: 357,900 Euro
Purpose of research: Applied research

In wide parts of Europe, Asia and America, the furoviruses soil-borne cereal mosaic virus (SBCMV) and soil-borne wheat mosaic virus (SBWMV) as well as the bymovirus Wheat spindle streak mosaic virus (WSSMV) cause significant yield losses in winter wheat. The only possibility to avoid infection and yield losses caused by these viruses is the cultivation of resistant varieties. Aim of this project is therefore mapping the WSSMV/SBCMV resistance, saturating the respective genome regions with molecular markers, developing molecular markers for effective marker associated selection, and gaining information on possible co-location of virus resistance genes with those already isolated in barley. To achieve these aims, different DH populations will be phenotyped for virus resistance at different locations and genotyped by using high-throughput techniques like the 90k iSelect Chip and genotyping-by-sequencing (GBS). Based on the results, the respective genes and QTL are mapped. Utilizing information on synteny, these genome regions will be saturated with markers. The processing of this project conducts in cooperation between the JKI-RS, where the genotyping and development of markers undertake, and the JKI-EP, that is responsible for phenotypic assessment of virus resistance in the DH lines populations. The assessment of the resistance to soil-borne viruses in wheat can be taken by different methods. It was prove that genotypes with virus resistance after naturally virus transmission by the vector Polymyxa graminis could be infected by mechanically leaf inoculation under climate chamber conditions. For this reason the phenotypic assessment of the wheat DH lines will be tested in three German and in one French infested fields. The rating of resistance carried out by serological detection of virus infection in the leaves of field plants by DAS-ELISA.

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