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Collaborative project: Screening for Wheat dwarf virus resistance / tolerance in the wheat gene pool and identification of QTL using association mapping - subproject 2

Project


Project code: 2814602913, JKI-RS-08-3383
Contract period: 01.08.2014 - 30.09.2017
Budget: 279,387 Euro
Purpose of research: Experimental development

Aims of this project are screening a wheat gene pool (consisting of Triticum aestivum gene bank accessions and breeding material, different species like T. monococcum, T. dicoccum, Thinopyrum intermedium and lines developed from this material) for genotypes with wheat dwarf virus tolerance/resistance, selecting WDV tolerant genotypes as source for wheat breeding programmes and identification of genome regions which are involved in WDV tolerance by genome wide association studies. The work schedule of this project includes the evaluation of a wheat gene pool with artificial and natural infection with wheat dwarf virus in field trials for 3 years, the phenotypic characterization of gene bank accessions, crossings of WDV tolerant genotypes with current varieties and breeding material followed by the production of DH lines. The development of molecular markers will be carried out using the 90k iSelect Chip and association genetics studies and is the requirement for effective selection for WDV tolerance/resistance in further wheat breeding programmes.

Due to global warming insect transmitted viruses like Wheat dwarf virus (WDV), will become more important in the future. The project therefore aims at screening Triticum aestivum gene bank accessions and breeding lines, related species like T. monococcum, T. dicoccum, and lines developed from crosses of these with T. aestivum for WDV resistance in order to indentify lines suited to be used in wheat breeding. Furthermore, by using genome-wide association studies the project aims to identify genome regions involved in WDV resistance in order to facilitate marker based selection for WDV resistance. 500 genotypes were tested for WDV tolerance by artificial inoculation with viruliferous leafhoppers (Psammotettix alienus). The same set of genotypes was tested in a field trial in Žabcice (Czech Republic) to identify WDV-resistant wheat genotypes under natural infection. Five accessions had an average infection rate 60% compared to control plants (1- or 2-year´s data). Among these, the Triticum aestivum accession “WDV_06” with a low mean infection rate (9.5%) and a high relative grain yield of 69% was identified and crossed with the varieties Faustus, Pontius and Moschus for the development of DH-lines (SRes) and with Reform for SSD-production (RAGT) in spring 2016. A subset of 250 hexaploid genotypes differing in WDV resistance level were genotyped with the 15k iSelect chip (Trait Genetics) for genome-wide association studies.

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