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Imprint
Collaborative project: Quantitative and qualitative detection of wheat and barley bunt diseases (Tilletia spp., Ustilago nuda) by means of biotechnolgy methods (q-PCR, LAMP-technology). Subproject: Development of LAMP-based quick tests for the determination of bunt diseases (Tilletia spp., Ustilago nuda) and population genetic studies on T. caries and T. controversa
Project
Project code: 2812NA128, JKI-EP-08-2253
Contract period: 01.10.2014
- 30.06.2019
Budget: 239,203 Euro
Purpose of research: Applied research
In this project highly efficient and economically viable methods will be developed to detect seedborne smut fungi (Tilletia spp., Ustilago nuda) in wheat and barley. With the help of these methods we are not only aiming at individually and unambiguously identifying the pathogens, also in mixed contaminations of seed lots, but we are also aiming at quantifying the contamination level of seed lots by these fungi. To reach these goals molecular biological methods, based on q-PCR and LAMP, respectively, will be developed and optimised. These methods will be internationally validated and then submitted to the International Seed Testing Association (ISTA) for implementation in the handbook “International Rules for Seed Testing”. In the first year q-PCR methods will be developed and optimised at the Landesanstalt für Landwirtschaft (LfL) while LAMP will be established at the Julius Kühn-Institut (JKI). As far as possible primer development and validation of the methods will be performed jointly to take advantage of potential synergies. In the second year validation studies in both institutions, LfL and JKI, will focus especially on the accuracy and robustness of identification of the different smut fungi and on their quantification. Several DNA-isolation-methods will be tested and potential cross-reactions of the developed methods with other fungal cereal parasites will be investigated. In the third year, more validation studies covering especially reproducibility, and repeatability will be performed at the LfL. Multiplexing of the tests will also play an important role. At JKI population structure of selected T. caries and T. controversa populations will be studied using already published and newly developed molecular markers (microsatellites) for these fungi.
Section overview
Subjects
- Crop Protection
- Biotechnology
Framework programme
Federal Organic Farming Scheme and other forms of sustainable agriculture (BÖLN)
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
