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Development of methods for PCR-based direct detection of three sugar beet viruses in soil samples and for typing of BNYVV isolates to ensure production of healthy sugar beets for bioenergy production
Project
Project code: JKI-EP-08-2219
Contract period: 01.01.2015
- 31.12.2018
Purpose of research: Applied research
Keywords: BNYVV, plant pathogen diagnosis, PCR-based quick method
Beet necrotic yellow vein virus (BNYVV) is the major factor causing Rhizomia disease of sugar beet, however Beet soil-borne virus (BSBV) and/or Beet virus Q (BVQ) are frequently found in infected plants, too. Almost nothing is known so far about their biological role and economic impact on this crop. The ubiquitous soil-borne protist Polymyxa betae is the vector for all three viruses which can keep their infectivy in the resting spores for many years. The projects aims to replace the laborious and time-consuming procedure to determine the infestation rate (BNYVV) of soil samples by a rapid and sensitive PCR-based method allowing the simultanous detection of BNYVV, BSBV, and BVQ. Moreover, a qPCR approach to quantify the BNYVV content in soil samples should be developd and the procedure for pathotyping of BNYVV isolates has to be optimized. Finally, the proportion between the diferent genome components of BNYVV has to be quantified under various conditions (cultivar, root/upper part, soil type etc.)
Section overview
Subjects
- Crop Protection
- Biotechnology
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
