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Interaction of foreign compounds, their metabolism, dietary fat and inflammatory processes in the liver
Project
Project code: BfR-LMS-08-1322-625
Contract period: 01.01.2015
- 31.12.2016
Purpose of research: Basic research
Non-alcoholic fatty liver disease, imbalanced food intake and disturbances of metabolism are continuously increasing in western countries. Commonly used in vitro cell systems aimed to analyze effects of foreign substances do not reflect this issue. A better understanding of the interplay of nuclear receptor-activating food constituents with lipid metabolism and inflammation-related processes, as well as an analysis of the consequences of these interactions for the xenobiotic-induced effects is necessary. This is subject of the research project 1322-625, within which this project is embedded. The project is thus aimed to contribute to a better understanding of the (patho-)physiological consequences of the uptake of different food components and the implications of certain conditions of the liver (steatosis, inflammation) for the metabolism of exogenous substances; by doing so, the project will improve the predictivity of in vitro studies in assessing the effects of food-borne substances in liver. According to schedule, using a mass spectrometry-based approach 288 samples from HepaRG or HC-AFW1 cells were analyzed for the activity of 7 important xenobiotic-metabolizing enzymes from the family of cytochrome P450-dependent monooxygenases, depending on treatment with xenobiotics, fatty acids and mediators of inflammation. Furthermore, the expression of 96 genes was analyzed by PCR array. Processed data were transmitted to the BfR as an excel spreadsheet. Data analysis was performed as part of the research project 1322-625.
Section overview
Subjects
- Physiology of Nutrition
