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Imprint
Hemoglobin adducts as biomarkers of internal exposure to genotoxic compounds
Project
Project code: BfR-LMS-08-1322-602
Contract period: 01.03.2014
- 31.12.2016
Purpose of research: Applied research
Aim of the project is to establish and apply methods for the detection of hemoglobin adducts as biomarker of internal exposure to genotoxic compounds. In a first step, methods will be established in order to qualitatively characterise the formation of hemoglobin adducts by means of example compounds such as glycidol or 3-MCPD, and to allow quantitative detection. In a second step, these methods will be used to detect the internal exposure of a group of people and to monitor the formation of specific hemoglobin adducts in a human study. Results of the project will contribute to a better recording of the internal exposure to compounds with actual relevance for risk assessment and will be valuable for risk characterisation of these substances.
Aim of the project is to establish and to use methods for the determination of hemoglobin adducts as biomarkers for the internal exposure in regard to genotoxic compounds. As a prerequisite for this project human hemoglobin was characterized by mass spectroscopic methods within the year of report. So far, a sequence coverage of 75 procent was achieved. A published method for the quantification of acrylamide-hemoglobin adducts was established in our laboratory and was extended to the detection of glycidol-hemoglobin adducts. Moreover, the supposed formation of hemoglobin adducts of 3-monochloropropanediol (3-MCPD) and 2-monochloropropanediol (2-MCPD) was examined, however, the results obtained so far indicate that these two compounds do not form hemoglobin adducts.
Section overview
Subjects
- Biotechnology
- Toxicology
