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Determination of glycidyl- and MCPD-fatty acid esters in infant formula Analysis by different methods
Project
Project code: BfR-SiN-08-1329
Contract period: 01.03.2016
- 31.08.2016
Purpose of research: Applied research
Different methods are described in literature to detect MCPD- and glycidyl-fatty acid esters in fat and oil. In the meantime three indirect methods of analysis, that means hydrolysis of the glycidyl-fatty acid esters followed by transformation of glycidol into a more stable compound are validated by AOCS in method validation studies. Further one LC-MS method for the analysis of glycidyl-fatty acid esters was validated by AOCS. For the analysis of fat containing foodstuffs up to now no method is validated in a comparable study. Due to the diversity of fat containing foodstuffs different method of extraction are likely to be required. In the Federal Institute for Risk Assessment for the analysis of infant formula the extraction of fat by accelerated solvent extraction was chosen. The extracted fat was analysed by an indirect method using acid hydrolysis of the fatty acid esters (Joint AOCS/JOCS Official Method Cd 29a-13). For the analysis of infant formula “1” and “Pre”, infant formula from seven different producers were selected. From each product 5 different lots, altogether 70 samples were analysed. This study was completed at the end of 2013. During the analysis of samples it was found that the fat extraction efficiency was only 80 % in some samples. Therefore the method of fat extraction was modified and 2014 to 2015 the samples of infant formula were analysed again. In all lots of two samples from one producer the detected concentrations of glycidyl-esters were significantly above the concentrations found 2013. The analysis with a second method (different type of hydrolysis) resulted in concentrations comparably with those detected in 2013. Also the analysis of the glycidyl-fatty acid esters in these samples with LC-MS confirmed the lower concentrations. To ensure these results the analysis by a second laboratory is urgently required.
Section overview
Subjects
- Food Chemistry
- Biotechnology
