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Mapping of terpene synthase genes and development of molecular markers for volatiles of carrot
Project
Project code: JKI-ZGO-08-4178
Contract period: 01.07.2016
- 30.06.2018
Purpose of research: Applied research
Terpenes are a highly diverse class of organic substances involved in different biological processes. So, specific combinations of terpenes are important flavour compounds of fruits and vegetables. Pollinating insects are attracted by terpenes produced by flower plants. Others protect plants by deterring herbivores and attracting predators and parasites of herbivores. They are constitutively present or will be induced as signal substances by stress factors. The great diversity, mainly of monoterpenes (C10) and sesquiterpenes (C15), results from different members of the terpene synthase gene family, each responsible for the production of one or more individual terpenes. Monoterpenes and sesquiterpenes are volatile organic compounds (VOCs) which can be separated and semi-quantified by HS-SPME-GC-FID. In a former project 10 putative monoterpene synthases, 10 putative sesquiterpene synthases and 10 other key enzymes of the two main terpene biosynthesis pathways DXP (in chloroplasts) and MVA (in cytosol) have been identified by bioinformatic gene mining. These 30 candidate genes of the terpene metabolism will be used for PCR amplification for both parents of a segregating carrot F2 progeny to identify useful polymorphisms (InDels, CAPS, SNPs). For genotyping respective SNPs should be used Competetive Allel Specific PCR (KASP)- technique to localize the candidate genes on the nine chromosomes of Daucus carota L. In parallel VOCs of all 320 F2 plants of the segregating progeny will be quantified for roots as well as for leaves. Aim of the project is to develop functional molecular markers for some important genes of the terpene metabolism of carrot on the basis of associations between candidate genes and the content of each VOC. Additionally semi-quantification of the VOCs in the root as well as in the leaves will provide the chance to get first results on tissue-specific regulation of these terpenes and their secretion into the atmosphere or into the rhizosphere.
Section overview
Subjects
- Plant Breeding
- Arboriculture
- Vegetable Gardening
Framework programme
Funding programme
Excutive institution
Institute for Breeding Research on Horticultural Crops (JKI-ZG)
