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Establishment of a stem cell-based system for the systematic testing of negative side-effects of drugs on human cardiomyocytes
Project
Project code: BfR-ZEBET-08-1329-471
Contract period: 01.09.2011
- 31.08.2014
Purpose of research: Applied research
Systematic optimization of the directed differentiation of human pluripotent stem cells into cardiomyocytes; characterization of the resulting cardiomyocytes in comparison to human heart tissue; establishment of a cardiotoxicity drug testing platform based on extracellular field potential recordings using multielectrode arrays; evaluation of the platform on disease models based on patient-specific stem cells.
Directed differentiation of hPSCs into cardiomyocytes was successfully optimizied, with efficiencies up to 90%; hPSC-CMs maturate in vitro upon long-term cultivation; multielectrode arrays allow for simple electrophysiological characterization of the cells and for evaluating drug effects on these; hPSC-CM disease model of Jervell and Lange Nielsen syndrome recapitulates disease phenotype of the underlying patient; pharmacological drug testing in these models is feasible.
Section overview
Subjects
- Biotechnology
- Toxicology
Framework programme
Funding programme
Excutive institution
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)