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Establishment of a stem cell-based system for the systematic testing of negative side-effects of drugs on human cardiomyocytes

Project

Food and consumer protection

This project contributes to the research aim 'Food and consumer protection'. Which funding institutions are active for this aim? What are the sub-aims? Take a look:
Food and consumer protection


Project code: BfR-ZEBET-08-1329-471
Contract period: 01.09.2011 - 31.08.2014
Purpose of research: Applied research

Systematic optimization of the directed differentiation of human pluripotent stem cells into cardiomyocytes; characterization of the resulting cardiomyocytes in comparison to human heart tissue; establishment of a cardiotoxicity drug testing platform based on extracellular field potential recordings using multielectrode arrays; evaluation of the platform on disease models based on patient-specific stem cells.

Directed differentiation of hPSCs into cardiomyocytes was successfully optimizied, with efficiencies up to 90%; hPSC-CMs maturate in vitro upon long-term cultivation; multielectrode arrays allow for simple electrophysiological characterization of the cells and for evaluating drug effects on these; hPSC-CM disease model of Jervell and Lange Nielsen syndrome recapitulates disease phenotype of the underlying patient; pharmacological drug testing in these models is feasible.

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Subjects

Framework programme

BMEL Frameworkprogramme 2008

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