BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
ZEBET is the 'Centre for Documentation and Evaluation of Alternative and Complementary Methods to Animal Experiments 'at the BfR. It was founded in 1989 with the aim to restrict the use of animals for scientific purposes to the absolute minimum and to develop alternatives to animal testing. ZEBET since 1994 is composed by a committee to advise representatives of science, industry, animal welfare organizations and state agencies. The goal of the scientific animal welfare will be achieved through the 3Rs principle: 1) Replace - avoidance of animal testing through the use of alternative methods 2) Reduce - reduce the number of animals to the minimum 3) Refine - reducing the suffering of animals ZEBET researched, developed and validated in our laboratory, alternative methods based on the 3R principle, mainly in the range of statutory, toxicological testing. She has achieved particular success in the development of alternative methods of field 1) the phototoxicity 2) the toxicity of skin and eye 3) the developmental toxicity using embryonic stem cells. This method examines the potential of a substance, growth and development of organisms affecting.
Parent institution:
Federal Institute for Risk Assessment (BfR) Details of BfR
Address
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
Diedersdorfer Weg 1
12277 Berlin
Berlin
Phone: +49-(0)30-8412-4300
Fax: +49-(0)30-8412-4970
email: zebet(@)bfr.bund.de
Activities:
- Research
Focus of Research:
- Alternative and Complementary Methods to Animal Testing
Coordinated projects
- Best Search Practice on Animal Alternatives - A Guide for Untrained Database Users Details of Best Search Practice on Animal Alternatives - A Guide for Untrained Database Users
- Biotechnological derived skin models for the cutaneous uptake of steroid hormone formulations Details of Biotechnological derived skin models for the cutaneous uptake of steroid hormone formulations
- Characterisation and prevalidation of a new human cornea equivalent model for the evaluation of ocular toxicity Details of Characterisation and prevalidation of a new human cornea equivalent model for the evaluation of ocular toxicity
- Computerised risk assessment of dermal absorption of active agents Details of Computerised risk assessment of dermal absorption of active agents
- Construction and Prevalidation of V79 Pathway Cell Lines: Heterologous Co-Expression of human Cytochrom P450 1A2 and polymorphic forms of human N-Acetyltransferase in V79 Chinese Hamster Cells for in vitro-studies on toxicity and metabolism of chemicals and drugs. Details of Construction and Prevalidation of V79 Pathway Cell Lines: Heterologous Co-Expression of human Cytochrom P450 1A2 and polymorphic forms of human N-Acetyltransferase in V79 Chinese Hamster Cells for in vitro-studies on toxicity and metabolism of chemicals and drugs.
- Development and evaluation of alternative methods to replace animal experiments (NIH-Test) for the potency measure of vaccines against rabies Details of Development and evaluation of alternative methods to replace animal experiments (NIH-Test) for the potency measure of vaccines against rabies
- Development and Validation of an Inductive Database for the Prediction of Carcinogenic Effects of Chemicals Details of Development and Validation of an Inductive Database for the Prediction of Carcinogenic Effects of Chemicals
- Development of a biotransformation system for the metabolic activation of validated in vitro systems to study embryotoxicity Details of Development of a biotransformation system for the metabolic activation of validated in vitro systems to study embryotoxicity
- Development of a novel approach in hazard and risk assessment of reproductive toxicity by a combination and application of in vitro, tissue and sensor technologies - ReProTect Details of Development of a novel approach in hazard and risk assessment of reproductive toxicity by a combination and application of in vitro, tissue and sensor technologies - ReProTect
- Development of a single cell model of adult cardiomyocytes as a replacement method of animal experiments? Optical measurements of excitation-contraction coupling under the influence of test substances Details of Development of a single cell model of adult cardiomyocytes as a replacement method of animal experiments? Optical measurements of excitation-contraction coupling under the influence of test substances
- Development of an in vitro model (skin culture from slaughtered animals) to study the influence of compounds on wound healing. Details of Development of an in vitro model (skin culture from slaughtered animals) to study the influence of compounds on wound healing.
- Development of bioreactors for in vitro testing of biological heart valves for reduction of animal experiments Details of Development of bioreactors for in vitro testing of biological heart valves for reduction of animal experiments
- Development of methods for the isolation and analysis of Yessotoxin Details of Development of methods for the isolation and analysis of Yessotoxin
- Development of Predictive In Vitro Test for Developmental Neurotoxicity testing Details of Development of Predictive In Vitro Test for Developmental Neurotoxicity testing
- Embryonic stem cell-based novel alternative testing strategies (ESNATS) Details of Embryonic stem cell-based novel alternative testing strategies (ESNATS)
- Equine precision cut lung slices as an in vitro model of obstructive airway diseases for the identification and characterization of drug targets Details of Equine precision cut lung slices as an in vitro model of obstructive airway diseases for the identification and characterization of drug targets
- Establishment of normal human neural progenitor cells as an in vitro screening model for the evaluation of the neurotoxic potential of chemicals Details of Establishment of normal human neural progenitor cells as an in vitro screening model for the evaluation of the neurotoxic potential of chemicals
- Estimating the risk of substances by spatial multi-biomarker profiling of 3D tissue cultures Details of Estimating the risk of substances by spatial multi-biomarker profiling of 3D tissue cultures
- Evaluation of Alternative Methods to Animal Experiments in Life Sciences Details of Evaluation of Alternative Methods to Animal Experiments in Life Sciences
- Further development of a 3D-model of substance distribution in the cochlea after round window application Details of Further development of a 3D-model of substance distribution in the cochlea after round window application
- Gastric cell lines to substitute native cells of the gastric gland to study acid secretion in special consideration of preclinical studies Details of Gastric cell lines to substitute native cells of the gastric gland to study acid secretion in special consideration of preclinical studies
- HPCT-1E3 hepatocytoma cultures for determination of cytotoxicity, toxicokinetic interactions and cholestatic effects, pre-validation study Details of HPCT-1E3 hepatocytoma cultures for determination of cytotoxicity, toxicokinetic interactions and cholestatic effects, pre-validation study
- Improvement of an in vitro stem cell assay for developmental toxicity: Establishing molecular endpoints of tissue specific development Details of Improvement of an in vitro stem cell assay for developmental toxicity: Establishing molecular endpoints of tissue specific development
- Improvement of the embryonic stem cell test (EST) using neural marker genes for testing the developmental neurotoxicity in vitro Details of Improvement of the embryonic stem cell test (EST) using neural marker genes for testing the developmental neurotoxicity in vitro
- Improving the gain of transgenic mouse embryos for cryopreservation Details of Improving the gain of transgenic mouse embryos for cryopreservation
- In vitro exposure of lung cells with aerosol at the air-liquid-layer as an alternative for inhalation experiments with animals Details of In vitro exposure of lung cells with aerosol at the air-liquid-layer as an alternative for inhalation experiments with animals
- Joint Project: Development of an in vitro embryo toxicity tests with mouse embryonic stem cells: Subproject 1 - Analysis of embryotoxic effects in the light of new endpoints and metabolism using an expanded selection of materials Details of Joint Project: Development of an in vitro embryo toxicity tests with mouse embryonic stem cells: Subproject 1 - Analysis of embryotoxic effects in the light of new endpoints and metabolism using an expanded selection of materials
- Joint project: Pluripotent stem cells in automated prediction of developmental osteotoxizität. Subproject 3: Development and establishment of a Osteoblsten differentiation assays Details of Joint project: Pluripotent stem cells in automated prediction of developmental osteotoxizität. Subproject 3: Development and establishment of a Osteoblsten differentiation assays
- List of chemicals with missing IC 50-values and/or LD 50-values Details of List of chemicals with missing IC 50-values and/or LD 50-values
- Normal human neural progenitorcells as a screening model for substances that are toxic for neural development. Details of Normal human neural progenitorcells as a screening model for substances that are toxic for neural development.
- Optical probe technique for the assessment of multidrug resistance profiles in 3-dimensional in vitro tumor models Details of Optical probe technique for the assessment of multidrug resistance profiles in 3-dimensional in vitro tumor models
- Preparation for external prevalidation: Optimization of the internally validated and standardized method for quantitation of angiogenesis and antiangiogenesis in vitro by determination of the angiogenic standard of the cells to be used Details of Preparation for external prevalidation: Optimization of the internally validated and standardized method for quantitation of angiogenesis and antiangiogenesis in vitro by determination of the angiogenic standard of the cells to be used
- Prevalidation of human cornea model based on immortalized corneal cell lines as in vitro alternative to the Draize eye test. Details of Prevalidation of human cornea model based on immortalized corneal cell lines as in vitro alternative to the Draize eye test.
- Refinement of the LCSA (loose-fit coculture-based sensitization assay) Details of Refinement of the LCSA (loose-fit coculture-based sensitization assay)
- Replacement of fetal calf serum in the embryonic stem cell test (EST) – Standardization of culture conditions for proliferation and differentiation of embryonic stem cells of the mouse Details of Replacement of fetal calf serum in the embryonic stem cell test (EST) – Standardization of culture conditions for proliferation and differentiation of embryonic stem cells of the mouse
- Replacement of transgenic animal models for determination of in vivo mutations by refinement of regulatory animal studies with the technique of the Random Mutations Capture Assay Details of Replacement of transgenic animal models for determination of in vivo mutations by refinement of regulatory animal studies with the technique of the Random Mutations Capture Assay
- siRNA technology in mammalian cells as alternative of knock-out mice: Functional investigations of proteins of the Myc-Miz-1-TopBP1-network (translation). Details of siRNA technology in mammalian cells as alternative of knock-out mice: Functional investigations of proteins of the Myc-Miz-1-TopBP1-network (translation).
- Studies on the anti-oxidative responses of lung cells as an endpoint for the assessment of aerosols after exposure at the air-liquid interface Details of Studies on the anti-oxidative responses of lung cells as an endpoint for the assessment of aerosols after exposure at the air-liquid interface
- The isolated perfused bovine udder as an in vitro model for the evaluation of sensibilisation/sensitisation induced by chemicals Details of The isolated perfused bovine udder as an in vitro model for the evaluation of sensibilisation/sensitisation induced by chemicals
- Use of transgenic animals in biomedical research in Germany - survey 2001 to 2003 and perspectives to change research to non-animal test methods Details of Use of transgenic animals in biomedical research in Germany - survey 2001 to 2003 and perspectives to change research to non-animal test methods
Involved in research projects:
- Characterization of metabolizing enzymes and biotransformation of selected test substances in human in-vitro skin models to identify an optimal test system for dermato-toxicological and exposure-related studies Details of Characterization of metabolizing enzymes and biotransformation of selected test substances in human in-vitro skin models to identify an optimal test system for dermato-toxicological and exposure-related studies