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Relevancy: 100%
Biogenesis and maintenance of plant vacuoles (Project)
Vacuoles are major storage organelles and they are important for seed maturation, fruit development and rhizome growth. They are also essential for plant growth by contributing to expansion growth. In spite of their apparent importance, vacuole biogenesis and maintenance in plants are not yet well explored and understood. In the proposed project, we want to elucidate the molecular mechanisms...
Funding period: 2011 - 2015
Relevancy: 100%
Programmed cell death in Arabidopsis: Function of KDELpeptidases in development and pathogen defense (2) (Project)
Programmed cell death (PCD) is a prerequisite for successful development and yield formation of crop plants. Plant PCD is effected by a group of cysteine endopeptidases with a C-terminal KDEL endoplasmatic reticulum (ER) retention signal. We want to elucidate their role in developmental cell death, especially in micro- and macro-gametogenesis, in seed and silique maturation, as well as in defence...
Funding period: 2011 - 2015
Relevancy: 100%
Comparison of amino acid supply mechanisms between natural and nematode-induced sink tissues in Arabidopsis (Project)
We want to identify amino acid transporters that supply sink tissues of Arabidopsis thaliana with essential amino acids. For this purpose we will use plants, which are impaired in the synthesis of lysine or branched chain amino acids in the developing seed, an endogenous sink tissue, and giant cells induced by root-knot nematodes, an induced sink tissue. Microarrays will be used to identify...
Funding period: 2011 - 2015
Relevancy: 100%
Functional characterization of Pathogenesis-Related10 (PR10) genes (Project)
We propose to carry out ligand binding and protein-protein interaction studies to test whether PR10 proteins function in metabolite sensing and signaling. We will try to restore the anthocyanin biosynthetic pathway in Fra a deficient genotypes. These lines and others will be used to elucidate more details of the role of PR10s during infection with pathogens. Finally, we want to test the...
Funding period: 2011 - 2015
Relevancy: 100%
Cross talk of ABA signalling with auxin and ethylene responses (Project)
The phytohormone abscisic acid (ABA) plays a central role in stress adaptation of plants. Abiotic signals such as drought and cold as well as biotic interactions lead to an activation of the ABA signal pathway. The ABA-mediated responses including regulation of stomata and growth interfere with other signal pathways of phytohormones. The integration of various endogenous signals is largely not...
Funding period: 2011 - 2015
Relevancy: 100%
Genetic analysis of complex traits in maize: major components of drought tolerance and growth-related traits (Project)
We will characterize genomic regions involved in drought tolerance in maize using next generation resequencing. These regions will be targeted in an introgression library segregating for drought tolerance. Target genes involved in the drought stress response will be analyzed in detail and natural allelic variation in target genes will be investigated in a panel of diverse maize lines. An in...
Funding period: 2011 - 2015
Relevancy: 100%
Genetic dissection of arbuscular mycorrhiza development (Project)
This project aims at a molecular understanding of the development and function of arbuscules, which form the interface of the ubiquitous arbuscular mycorrhiza (AM) symbiosis, and are of central relevance for symbiotic nutrient supply. We propose map-based cloning of RED and DIS, two loci required for arbuscule development in Lotus japonicus and the subsequent functional characterization of the...
Funding period: 2011 - 2015
Relevancy: 100%
Structural and functional dynamics of remorin proteins during plant pathogen interactions (Project)
We have shown that remorin proteins are candidate facilitators of signalling cascades and signalling complex assembly. Here, we will functionally characterise two remorins from Arabidopsis thaliana that are involved in plant immunity. Analysis of plant mutants and transgenic lines in response to bacterial and fungal pathogens will be complemented by the identification of proteins interacting with...
Funding period: 2011 - 2015
Relevancy: 100%
Biotechnological applications of natural defense mechanisms (Project)
We will perform QTL-mapping to determine elements and factors required for the regulation of the Bx-gene cluster. Candidate genes identified by fine mapping will be functionally tested (co-regulation, mutant analysis, transgenic approaches). To get insight into the consequences of the transfer of an alien secondary metabolic pathway, we will analyse transgenic A. thaliana expressing combinations...
Funding period: 2011 - 2015
Relevancy: 100%
Systemic acquired resistance in plants – an -omics approach to pathogen defense signalling (Project)
This project aims to identify systemic acquired resistance (SAR) signalling components in Arabidopsis thaliana and a mutant, eds1, which is incapable of generating or transmitting SAR signals. The EDS1-dependent peptide, lipid, and polar small molecule content of extracts of induced plants will be identified by various mass spectrometry-based techniques. The defence-inducing potential of thus...
Funding period: 2011 - 2015
