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Biodiversity of microorgansisms in forest soils: Pilot study for an additional investigation to the 2nd Soil Survey
Project
Project code: BFH-II-02-39
Contract period: 01.07.2007
- 30.06.2008
Purpose of research: Applied research
Chemical and physical parameters of soil samples and forest characteristics were assessed in an area-wide grid in the 2nd Soil Survey in Germany. The biodiversity of microorganisms in forest soils will be characterised as an accompanying investigation.
The cultivation-independent methods based on DNA analyses are favourable because of the characterisation of microbial communities without the modificatory selection pressure of artificial agars. In these techniques, the 16S-rDNA of Bacteria and Archaea and the ITS1-5.8S-Its2 region of fungi are especially suitable for a taxonomic evaluation.
In a first pilot study the reproducible extraction of DNA from forest soil samples with a high content of humic acids will be optimised and the T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) will be established.
Section overview
Subjects
- Forestry
- Soil science